2018-09-25 13:59:332025-05-28 13:30:48
locus
BSU39110
BSU_39110
geneLength
1437
1440
outlinks
bsu
BSU39110
BSU_39110
The protein
Catalyzed reaction/ biological activity
RNA helicase
RNA helicase
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
The protein
Protein family
[SW|DEAD-box RNA helicases]
[SW|helicase family] (according to UniProt)
[SW|DEAD-box RNA helicases] (according to UniProt)
The protein
[SW|Domains]
Helicase domain 1: AS 1-203
Linker region: AS 204-212
Helicase domain 2: AS 207-368
RNA-binding domain: AS 404-479
[SW|Helicase ATP-binding domain] (aa 33-203) (according to UniProt)
[SW|Helicase C-terminal domain] (aa 214-374) (according to UniProt)
The protein
Structure
[PDB|2G0C] (RNA-binding domain, AA 404-479), [PDB|2HJV] (second domain, AA 207-368); [PDB|3MOJ] (RNA binding domain complexed with a fragment of 23S ribosomal RNA) [Pubmed|20673833]
[PDB|5IVL] (from Geobacillus stearothermophilus, corresponds to aa 1 ... 373, 44% identity) [pubmed|28238534]
[PDB|2G0C] (RNA-binding domain, AA 404-479)
[PDB|2HJV] (second domain, AA 207-368)
[PDB|3MOJ] (RNA binding domain complexed with a fragment of 23S ribosomal RNA) [Pubmed|20673833]
Biological materials
Expression vector
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1065 (spc), available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Dagmar Klostermeier], Biozentrum Basel, Switzerland [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
References
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1065 (spc), available in [SW|Jörg Stülke]'s lab
labs
[SW|Dagmar Klostermeier], Biozentrum Basel, Switzerland [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]